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. 2018 Jul 9;7(14):e009289. doi: 10.1161/JAHA.118.009289

Figure 12.

Figure 12

Mitochondrial fission inhibition by mdivi‐1 preserved mitochondrial respiration in D7‐Des overexpressed NRCs. A, Mitochondrial oxygen consumption rate (OCR) profile in NRCs treated with 5 μmol/L mdivi‐1 or vehicle for 24 hours in D7‐Des overexpressed cardiomyocytes. Arrow indicates the sequential addition of oligomycin (1 μmol/L), FCCP (4 μmol/L), and rotenone (0.5 μmol/L) plus antimycin A (0.5 μmol/L). OCR profile is expressed as pmol O2/min per μg of protein. Graph shows OCR under (B) baseline as well as with the addition of (C) oligomycin, (D) FCCP, and (E) rotenone plus antimycin A. Critical parameters of mitochondrial function including (F) reserve capacity, (G) ATP turnover, and (H) maximal respiration were significantly rescued by mdivi‐1 treatment in D7‐Des overexpressed NRCs. Bars represent mean±SEM. n=5 wells per group. P values were determined by Tukey's post hoc test. FCCP indicates carbonyl cyanide‐p‐trifluoromethoxy‐phenylhydrazone; NRCs, neonatal rat cardiomyocytes; NS, not significant.