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. 2018 Jul 23;8:252. doi: 10.3389/fcimb.2018.00252

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Copyright © 2018 Higgins, Heeb, Rampioni, Fletcher, Williams and Cámara.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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A delay in the timing of PphzA1 activation was observed when lasR was mutated in the pqsEind strain. The pqsEind strain increased the activity of PphzA1-lux (green line) but when lasR is mutated a 2 h delay in promoter activation is observed (blue line) compared with the pqsEind. When rhlR is deleted from the pqsEind strain PphzA1-lux activity is abolished (red line). The growth curves of the 3 mutants are also shown and this data is plotted on the Z axis. All strains were induced with 1 mM IPTG and promoter activities are normalized by cell density (A₆₀₀).