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PQS is unable to induce high levels of PphzA1 transcription in the absence of PqsE or RhlR. Maximal promoter activity of PphzA1-lux in the pqsEind strain and pqsEind strain carrying additional lasR or rhlR mutations in the presence or absence of IPTG and PQS. Promoter activities are normalized by cell density (A600).
