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. 2018 Jun 26;7(13):e008654. doi: 10.1161/JAHA.118.008654

Figure 4.

Figure 4

Carboxyl‐terminal modulator protein (CTMP) alleviates angiotensin II (AngII)–induced cardiomyocyte hypertrophy. A, Western blot analysis of CTMP expression in neonatal rat cardiomyocytes (NRCMs) infected with adenoviral short‐hairpin CTMP (AdshCTMP) and adenoviral CTMP (AdCTMP) and their controls (n=3 independent experiments). B, Representative images of NRCMs infected with adenoviral short‐hairpin RNA (AdshRNA), AdshCTMP, adenoviral green fluorescent protein (AdGFP), and AdCTMP treated with PBS or AngII for 48 hours (48h; green, α‐actinin; blue, nuclear; bar=40 μm). C and D, Quantitative analysis of the cell surface area of NRCMs infected with AdshRNA or AdshCTMP (C) and AdGFP or AdCTMP (D) treated with PBS or AngII for 48h (n>50 cells per group; **P<0.01 vs AdshRNA+PBS or AdGFP+PBS; # P<0.05 vs AdshRNA+AngII; ## P<0.01 vs AdGFP+AngII). E and F, Real‐time polymerase chain reaction analyses of the hypertrophic biomarkers atrial natriuretic peptide (Anp) and myosin heavy chain 7 (Myh7) in NRCMs infected with AdshRNA or AdshCTMP (E) and AdGFP or AdCTMP (F) treated with PBS or AngII (n=3 independent experiments; **P<0.01 vs AdshRNA+PBS or AdGFP+PBS; ## P<0.01 vs AdshRNA+AngII or AdGFP+AngII) by Tamhane's T2 analysis (C and E) or Bonferroni post hoc analysis (D and F).