Table 1.
Primer sequences and amplification efficiency of candidate reference genes.
| Gene symbol | Primer sequence (5′ to 3′) | Amplicon length (bp) | E (%) | r2 |
|---|---|---|---|---|
| ACT | F: CTCCCAGCCGAACTAACAAACC | 178 | 95.3 | 0.999 |
| R: CTTCTGTCCCATACCGACCATG | ||||
| EF | F: TCAGGCTGATTGTGCTGTCTTG | 199 | 96.7 | 0.999 |
| R: TTCGTTGACCACTTCGGTGTAA | ||||
| HIS | F: GCCATTCGTCGTCTCGCTCGTC | 106 | 95.9 | 0.999 |
| R: CACGGATCACATTCTCCAAGAAC | ||||
| PMP | F: TGGAGGACGGAAGATACGAG | 176 | 95.3 | 0.999 |
| R: TTGGGAGTGACCAGTGGAAC | ||||
| TUB | F: TGACAACGAAGCCCTTTACGAC | 200 | 99.3 | 0.999 |
| R: CAGGCATGAAGAAGTGGAGACG | ||||
| UBCE | F: ATCGCCTACGGTCTACGGGTAC | 124 | 95.9 | 0.999 |
| R: TAACGGCAGATAAATTGCTGGA | ||||
| UBQ | F: CTTCGTCTCCGTGGAGGTAT | 109 | 96.7 | 0.999 |
| R: GGATCTTGGCTTTGACATTCT | ||||
| 18S rRNA | F: TTCTGACCGTAAACGATGCCAACT | 169 | 93.5 | 0.999 |
| R: ATTAAGCCGCAAGCTCCACTCC | ||||