Table 2.
Expression stability ranking under different habitat conditions of the eight candidate reference genes using five algorithms.
| Gene | RefFinder |
geNorm |
NormFinder |
BestKeeper |
Delta Cq |
|||||
|---|---|---|---|---|---|---|---|---|---|---|
| Rank | GM | Rank | MV | Rank | SV | Rank | SD | Rank | SD | |
| TUB | 1 | 1.41 | 1 | 0.72 | 2 | 0.09 | 2 | 0.43 | 1 | 0.69 |
| 18S rRNA | 2 | 1.57 | 2 | 0.73 | 1 | 0.07 | 3 | 0.53 | 2 | 0.71 |
| UBCE | 3 | 3.22 | 3 | 0.97 | 3 | 0.44 | 4 | 0.59 | 3 | 0.89 |
| HIS | 4 | 3.31 | 5 | 1.01 | 5 | 0.50 | 1 | 0.32 | 5 | 0.98 |
| UBQ | 5 | 4.47 | 4 | 0.98 | 7 | 0.60 | 5 | 0.74 | 4 | 0.97 |
| EF | 6 | 6.19 | 6 | 1.03 | 4 | 0.45 | 7 | 0.97 | 6 | 1.01 |
| PMP | 7 | 6.48 | 7 | 1.09 | 8 | 0.82 | 6 | 0.92 | 7 | 1.03 |
| ACT | 8 | 8.00 | 8 | 1.13 | 6 | 0.57 | 8 | 1.04 | 8 | 1.06 |
Rankings were determined using the parameters GM, geometric mean; SV, stability value; MV, M-Value; and SD, standard deviation.