Table 5.
Expression stability ranking at different developmental stages of the eight candidate reference genes using five algorithms.
| Gene | RefFinder |
geNorm |
NormFinder |
BestKeeper |
Delta Cq |
|||||
|---|---|---|---|---|---|---|---|---|---|---|
| Rank | GM | Rank | MV | Rank | SV | Rank | SD | Rank | SD | |
| TUB | 1 | 1.00 | 1 | 0.88 | 2 | 0.17 | 1 | 0.61 | 1 | 0.80 |
| HIS | 2 | 1.86 | 2 | 0.92 | 1 | 0.09 | 3 | 0.78 | 2 | 0.83 |
| 18S rRNA | 3 | 3.08 | 3 | 0.99 | 3 | 0.38 | 2 | 0.64 | 3 | 0.87 |
| UBQ | 4 | 3.94 | 8 | 1.48 | 8 | 0.91 | 4 | 0.98 | 4 | 0.88 |
| UBCE | 5 | 5.32 | 4 | 1.07 | 4 | 0.45 | 7 | 1.17 | 5 | 0.89 |
| ACT | 6 | 5.42 | 6 | 1.35 | 7 | 0.82 | 6 | 1.16 | 6 | 1.17 |
| EF | 7 | 7.00 | 7 | 1.39 | 6 | 0.81 | 5 | 0.99 | 7 | 1.28 |
| PMP | 8 | 7.44 | 5 | 1.32 | 5 | 0.73 | 8 | 1.49 | 8 | 1.34 |
Parameters of rankings were determined using the parameters GM, geometric mean; SV, stability value; MV, M-Value; and SD, standard deviation.