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. 2018 Jul 23;9:911. doi: 10.3389/fphys.2018.00911

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Copyright © 2018 Pires, Marques, Encarnação, Abrantes, Marques, Laranjo, Oliveira, Casalta-Lopes, Gonçalves, Sarmento-Ribeiro and Botelho.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Analysis of cell viability and types of cell death induced in C2BBe1, LS1034, and WiDr cells after exposure to AA and 5-FU, oxaliplatin (Oxa) or irinotecan (Iri) alone or in combined therapy (CT) for 48 h. The results are represented in percentage (%) of viable cells (V), initial apoptosis (IA), late apoptosis/necrosis (LA/N) and necrosis (N). The results express the mean and standard error of at least three independent experiments, in duplicate. Statistically significant differences relative to control are marked with ^∗p < 0.05, ^∗∗p < 0.01 and ^∗∗∗p < 0.001.