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. 2018 Jul 23;9:911. doi: 10.3389/fphys.2018.00911

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Copyright © 2018 Pires, Marques, Encarnação, Abrantes, Marques, Laranjo, Oliveira, Casalta-Lopes, Gonçalves, Sarmento-Ribeiro and Botelho.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Analysis of BAX and BCL-2 expression and mitochondrial membrane potential (Δψ_m) after exposure of C2BBe1, LS1034 and WiDr cells to AA and Oxa alone or in CT for 48 h. (A) Mitochondrial membrane potential (Δψ_m) values are expressed as aggregates/monomers (A/M) and (B) apoptosis-related proteins are expressed as BAX/BCL-2 ratio for each condition and cell line. The decrease in the A/M ratio is directly correlated with decreased Δψ_m. All results express the mean and standard deviation of at least four independent experiments, in duplicate. Statistically significant differences are marked with ^∗p < 0.05, ^∗∗p < 0.01, and ^∗∗∗p < 0.001.