Table 2.

Oligonucleotides

Oligonucleotide	Sequence, 5′–3′
Mouse lipT2, forward (NdeI)	ATTCACATATGAGCCTGCCGGTGGTG
Mouse lipT2, reverse (SalI)	TATAGTCGACTTAGCTCGGGCTATCTTCGCTAATCAG
Human gcsH, forward (BspHI)	ATAATTCATGAGCGTGCGCAAATTCA
Human gcsH, reverse (HindIII)	TAGATAAGCTTTCACTCCTCAATGG
P1 priming site for E. coli lipB	TTTCCCCCACTTTTACTCATTCTCCACGGAGATGCCGTTTT
	GTATCAGGATAAAATTCgtgtaggctggagctgcttc
P2 priming site for E. coli lipB	AGATATTATGAGTAATGACCCAGTGTAAATTGGGCCA
	TTGATGTATGGAAcatatgaatatcctcctta
lipB 250 bp upstream, forward	AAGTGGTACAGCGCCATGCGC
lipB 250 bp downstream, reverse	TTGGAACGGAACGCTTTTGCTCACC