Fig. 2.

Polθ efficiently extends a primer/template with 3–4 bp at the 3′ end. (A) The DNA oligos used in the experiment. (B) DNA synthesis reactions with 100 nM Polθ, 100 nM DNA substrate, and 0.1 mM dTTP at 25 °C in the standard reaction buffer. (C) Quantification of the DNA products at 2.5 min and 10 min. Quantification is based on the intensity of each band relative to the sum of all bands (including all products and primer substrate). The y axis shows percentages of individual products of primer extensions by +1, +2, +3, and etc., and the unfilled region represents the unused primer substrate.