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Polθ is sensitive to the presence and location of mismatched base pairs. (A) The DNA substrates used in the experiment. (B) DNA synthesis reactions with 100 nM Polθ, 100 nM DNA substrate, and 0.1 mM dTTP at 25 °C in the standard reaction buffer. (C) Quantification of the DNA products at 5 min and 20 min.
