Fig. 2.

Disease activity monitored in the mdx^5cv degeneration reporter mice. (A) Representative examples of wild-type and mdx^5cv degeneration reporter strains imaged at 5 mo of age following tamoxifen injection at 3 mo of age. (B) BLI data from hindlimb muscles of both wild-type and mdx^5cv degeneration reporter mice before tamoxifen injection (3 mo of age; “Pre-tam”) and each month thereafter, up to 12 mo of age. Data represent the mean ± SEM; n = 12; ***P < 0.0001, relative to the respective control group. (C) Luciferase enzymatic activity from hindlimb muscles of degeneration reporter mice of different age ranges following tamoxifen injection at 2 mo of age. (D) Immunostaining for luciferase (red) and laminin (cyan) in gastrocnemius muscles of mdx^5cv degeneration reporter mice at different ages following tamoxifen injection at 2 mo of age. 4,6-diamidino-2-phenylindole appears as blue. (Scale bar: 50 µm.)