Fig. 3.

Surface plasmon resonance quantifies binding of CD52-Fc to HMGB1. Binding of CD52-Fc (A) or Fc control protein to HMGB1 (B), CD52-Fc to Siglec-10-Fc (C), and CD52-Fc with or without HMGB1 to Siglec-10-Fc (D). HMGB1 (100 µg/mL) or Siglec-10-Fc (25 µg/mL) were immobilized on CM5 dextran sensor chips. As a control for nonspecific binding, recombinant Box A was used in A and B and recombinant Fc in C and D. Data are representative of three experiments. Further details are provided in SI Appendix, SI Materials and Methods.