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. 2018 Jul 11;115(30):E7053–E7062. doi: 10.1073/pnas.1803909115

Fig. 6.

Fig. 6.

IBM phosphorylation by CK2 increases the affinity of POGZ and MLL1 for the LEDGF/p75 IBD. (A) Diagrams of POGZ and MLL1 constructs and mutants used in B and C. (B and C) WT or mutants of POGZ (B) and MLL1 (C) were titrated against FLAG-tagged LEDGF/p75 in AlphaScreen after preincubation with or without CK2. Error bars represent SDs calculated from independent measurements (n = 4). (D) HEK 293T cells were transfected with FLAG-tagged POGZ. The proteins were immunoprecipitated with anti-FLAG antibody in the absence (−) or presence (+) of selective CK2 inhibitor (TBB). The presence of FLAG-tagged POGZ and endogenous LEDGF/p75 was confirmed using Western blot. (E) Densitometry of Western blot bands. Data are plotted as the ratio of LEDGF/p75 over FLAG-POGZ, normalized by DMSO control (mean ± SD from n = 3 independent replicates). P values obtained from Student’s t test; ***P < 0.0001.