Fig. 2.

RalGAPB knockout dramatically activates glucose uptake into BAT. Blood and tissues were collected from 8-wk-old mice after 6-h fast unless indicated otherwise. (A) WB of lysates from indicated tissues. Active RalA (aRalA) was determined by pull-down with RalBP1 agarose. (B–D) In vivo tissue-specific 2-deoxy-d-glucose uptake. Blood glucose (C) and insulin (D) levels before the experiment; eight mice per group. (E and F) Representative WB (out of five experiments) (E) and Ponceau stain (F) of BAT subcellular fractions collected after an overnight fast. The data are presented as mean ± SEM; *P < 0.05.