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. 2018 Jul 9;115(30):E7149–E7157. doi: 10.1073/pnas.1802261115

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Fig. 4. — Th17 cells recognize a broader spectrum of bacterial antigens than antibodies. (A) Experimental design. Whole-cell lysate (WCL), cytosol (Cyto), and membrane (Mem) fractions, as well as outer membrane vesicles (OMVs) were isolated from NT127, Eagan, and 86-028NP. Hi, H. influenzae. (B) Reactivity of pooled immune sera from NT127-infected mice to different bacterial fractions was analyzed by ELISA. (C) Lung lymphocytes from NT127-infected mice were stimulated with different bacterial fractions, and IL-17 production by CD4 T cells was measured by ICS.