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Subcellular localization of TaNOXs. Analysis of the subcellular location was performed using rice protoplast transient transformation systems. The constructs of TaNOX7::eGFP, TaNOX10::eGFP, TaFRO4::eGFP, and TaNOX-like4::eGFP were all under the control of the constitutive 2 × 35S promoters (cauliflower mosaic virus [CaMV]) in pTF486 vectors; at least three monoclones were sent for sequencing upon gene cloning and vector construction for each gene.
