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. Author manuscript; available in PMC: 2019 May 17.
Published in final edited form as: Cell. 2018 Apr 26;173(5):1165–1178.e20. doi: 10.1016/j.cell.2018.03.072

Figure 1. Cohesin translocation requires ATP and Smc ATPases.

Figure 1

(A) Schematics representing the extrusion model of nuclear topology. Cohesin rings are loaded at Nipbl+ sites, extrude along DNA, and halt at CTCF+ anchor sites in the convergent orientation. (B) Venn diagram showing the distribution of Rad21 ChIP-Seq peaks relative to CTCF and Nipbl. (C) ChIP-Seq tracks of CTCF, Nipbl, and Rad21 in the presence and absence of ATP in activated B cells. (D) Histograms showing global Rad21 (left) or CTCF (right) occupancy at Nipbl+ loading sites in oligomycin-treated (black) and untreated (red) activated B cells. (E) ChIP-Seq profiles of transduced Smc3-biotag WT and ATPase mutants in activated B cells. (F) Occupancy of Smc3 WT and mutants at loading and anchor sites.