Skip to main content
. Author manuscript; available in PMC: 2019 May 17.
Published in final edited form as: Cell. 2018 Apr 26;173(5):1165–1178.e20. doi: 10.1016/j.cell.2018.03.072

Figure 3. Characterization of architectural stripes.

Figure 3

(A) Examples of stripes (black arrowheads) in Bcl6 and Basp1 domains in activated B cells. CTCF and Nipbl tracks are shown below. “a” single-stripe domain; “b” double-stripe domain; “c” conventional loop domain. (B) Bar graph illustrates the percentage of stripe anchors associated with CTCF binding motifs. The pie chart shows the percentage of motifs facing the stripe. Data are represented as mean ± SEM. (C) Violin plot shows insulation scores for stripe and stripe partner anchors. (D) Upper schematic depicts stripe formation at the Basp1 domain based on the extrusion model. Composite graph shows Nipbl profiles at loop domains displaying double (blue), left (red), or right stripes (green), or at no stripes (grey). (E) Hi-C matrices show the loss of stripes in ZF9-11 cells at loci where stripe anchors fail to recruit CTCF. (F) Molecular dynamic simulations of Hi-C data where cohesin is loaded near the left (first domain) or right anchor CTCF anchors (third domain).