Fig. 4.

The emerging role of KDM4D in the angiogenic progression of GIST cells. a GIST882 and GIST-T1 cells stably expressing vector or KDM4D were harvested, and expression of the indicated proteins was examined by Western blot. GAPDH served as the loading control. b GIST882 and GIST-T1 cells stably expressing vector or KDM4D were cultured with DMEM without serum overnight. Conditioned media were collected for ELISA assays to assess VEGFA secretion. ***p < 0.001 (c) GIST882 and GIST-T1 cells stably expressing ShNC or ShKDM4D were harvested and expression of the indicated protein was examined by Western blot. GAPDH served as the loading control. d GIST882 and GIST-T1 cells stably expressing ShNC or ShKDM4D were cultured with DMEM without serum overnight. Conditioned media were collected for ELISA assays to assess VEGFA secretion. ***p < 0.001 (e) Conditioned media from GIST882 and GIST-T1 cells stably expressing vector or KDM4D were incubated with HUVECs cells in 96-well plates. The tube formation was examined by the branching points per filed. ***p < 0.001 (f) Conditioned media from GIST882 and GIST-T1 cells stably expressing ShNC or ShKDM4D were incubated with HUVECs cells in 96-well plates. Tube formation was examined based on the number of branching points per field. ***p < 0.001