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. 2018 Jul 30;19:48. doi: 10.1186/s12863-018-0653-4

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© The Author(s). 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 3 — Subcellular localization and transcriptional activity assays of GhWRKY42. a Transient expression of the 35S-GhWRKY42::GFP construct in onion epidermal cells. b Transcriptional activity of GhWRKY42 in Y2HGold yeast cells. The ORF of GhWRKY42 was cloned into the pGBKT7 vector. The constructs were transformed into Y2HGold yeast cells and identified on SD/−Trp/−Leu and SD/−Trp/−Leu/-His/−Ade medium. PC, positive control (pGADT7-largeT+pGBKT7-p53); NC, negative control (pGADT7-largeT+pGBKT7-laminC); AD+BD-42, experimental group (pGADT7-largeT+pGBKT7-GhWRKY42)