Fig. 6.

Expression profiles of GhWRKY42 in different tissues and during leaf senescence in cotton. a Expression levels of GhWRKY42 in different tissues. b Expression profile of GhWRKY42 during leaf senescence in 15-, 25-, 35-, 45-, 55-, and 65-day-old leaves. RPKM, reads per kilobase per million mapped reads. c Expression level of GhWRKY42 in true leaves at different developmental stages. Stage 1, a fully expanded, young, non-senescent leaf; Stage 2, a fully expanded, mature, non-senescent leaf; Stage 3, a leaf in early senescence with < 25% leaf area yellowing; Stage 4, a leaf in mid-senescence with approximately 50% leaf area yellowing; Stage 5, a leaf in late senescence with > 75% leaf area yellowing. d Relative expression level of GhWRKY42 in the cotyledons of the CCRI 10 and Liao 4086 varieties. Cotyledon samples were collected weekly at eight different development stages, ranging from the flattened cotyledon stage to the completely aged stage. GhActin served as the reference gene. The bars represent the means ± SEs from three independent experiments