Table 2.
Effect of BPN-14136 treatment on concentration of visual cycle retinoids in mouse eyes
Recovered visual cycle retinoids were quantified after their extraction from dark-adapted (DA) and light-adapted (LA) mouse eyes as well as from the eyes collected immediately after exposure to photobleaching (PB) as described under “Experimental procedures.” The number of animals per treatment group is shown in parentheses. The effect of treatment within each group was analyzed using t tests. Statistical significance was determined using the Holm-Sidak method, with α = 0.05. Each retinoid group for a given light condition was analyzed individually, without assuming a consistent S.D. Data are represented as mean ± S.D. ns, p > 0.05; **, p ≤ 0.01; ***, p ≤ 0.001; ****, p ≤ 0.0001. RAL is retinaldehyde.
| Treatment group | 11-cis-RAL | All-trans-RAL | All-trans-retinyl palmitate |
|---|---|---|---|
| pmol/eye | pmol/eye | pmol/eye | |
| DA | |||
| BPN-14136 (n = 6) | 188.2 ± 27.6**** | 14.5 ± 3.3** | 46.6 ± 7.1ns |
| Control (n = 6) | 315.5 ± 18.8 | 21.1 ± 3.4 | 59.3 ± 13.5 |
| LA | |||
| BPN-14136 (n = 6) | 30.8 ± 7.5*** | 51.9 ± 7.0** | 56.6 ± 18.0** |
| Control (n = 6) | 120.4 ± 43.9 | 90.6 ± 28.8 | 96.2 ± 17.6 |
| PB | |||
| BPN-14136 (n = 6) | 16.9 ± 2.6** | 111.0 ± 7.7**** | 58.7 ± 5.3**** |
| Control (n = 6) | 27.1 ± 5.8 | 182.7 ± 17.5 | 112.4 ± 16.4 |