Figure 2.

Ovalbumin forms giant β-sheet–rich amyloid nanosheets. A, absorption spectra of Congo red with various aggregates. The aggregates were scanned in the 400–700-nm range. B, secondary structure determination of nanosheets by UV CD. Native ovalbumin and nanosheet (200 μg/ml) were scanned in the range of 200–250 nm. Data are representative of at least three independent experiments with similar observations. C, nanosheet formation as seen by transmission EM. a, 6-h ovalbumin; b, 12-h ovalbumin; c and d, 24-h ovalbumin (nanosheet). Various aggregates (100 μg) were coated onto the grid. OVA, ovalbumin; CR, Congo red. Images shown are those selected after similar observation of at least two independent experiments performed for each sample.