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. 2018 Jun 13;293(29):11470–11480. doi: 10.1074/jbc.RA118.001872

Figure 1.

Figure 1.

Role of endocytosis in CXCL12-instigated Akt phosphorylation. A, CXCR4 internalization was quantified by ELISA. HeLa cells transfected with HA-CXCR4 were treated with 80 μm dynasore or DMSO for 30 min followed by 10 nm CXCL12 or vehicle for 5 min. Bars represent the average percentage of CXCL12-induced receptor internalization from three independent experiments. Error bars represent the S.D. Data were analyzed by Student's unpaired t test. p value is indicated. B, immunoblot analysis of Akt and ERK-1/2 phosphorylation. Serum-starved HeLa cells were treated with 80 μm dynasore or DMSO for 30–60 min followed by 10 nm CXCL12 or vehicle for 5 min. Whole-cell lysates were analyzed by immunoblotting for the indicated proteins. Representative immunoblots from four independent experiments are shown. C and D, immunoblots were analyzed by densitometry. Bars represent the mean of the relative levels of pAkt-Ser473 (C) or pERK-1/2 (D) to DMSO- and CXCL12-treated samples. Error bars represent the S.D. Data were analyzed by two-way ANOVA followed by Tukey's multiple comparison test. p value is indicated.