Figure 4.

Role of endocytosis in CXCL12- or CXCL13-instigated Akt phosphorylation in WEHI-231 cells. A, CXCL12- or CXCL13-instigated Akt phosphorylation in WEHI-231 cells. Serum-starved WEHI-231 cells were treated with 80 μm dynasore (DYN) or DMSO for 30 min and then 10 nm CXCL12, 100 nm CXCL13, or vehicle (Veh) for 5 min at 37 °C. Whole-cell lysates were analyzed by immunoblotting for pAkt-Ser⁴⁷³ and Akt. B, immunoblots were analyzed by densitometry. Bars represent the mean from three independent experiments relative to cells treated with DMSO and CXCL12. Error bars represent the S.D.