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. 2018 Jun 5;293(29):11401–11414. doi: 10.1074/jbc.RA118.003616

Figure 5.

Figure 5.

Proximal E-box contributes ILEI promoter vemurafenib responsiveness. A, luciferase assay of various length ILEI promoter reporter constructs in 501-Mel melanoma cell lines. Black bars indicate control DMSO treatment and white bars indicate 24 h treatment with vemurafenib (1 μm). Values are normalized to DMSO treatment of the particular reporter construct. n = 3, mean ± S.D., p value was indicated by Student's t test as compared with vemurafenib treatment of the empty promoterless reporter. B, luciferase assay of WT and E-box mutant (CACGTG to CAAATG) ILEI promoter reporter constructs (−300/−80) in 501-Mel melanoma cell lines. Black bars indicate control DMSO treatment and white bars indicate 24 h treatment with vemurafenib (1 μm). n = 3, mean ± S.D., p value was indicated by Student's t test comparing vemurafenib to DMSO treatment.