Effect of endogenous USF-1 on ILEI expression.
A, PCR analysis for ILEI, tyrosinase, or GAPDH of 501-Mel melanoma cells 24 h after treatment with 0, 5, or 50 J/m2 of UV. B, qPCR analysis of ILEI in 501-Mel melanoma cells 24 h after treatment with 0, 5, or 50 J/m2 of UV. n = 3, mean ± S.D., * indicates p < 0.05 compared with 0 UV by Student's t test. C, qPCR analysis of ILEI in HMLE human mammary epithelial cells 24 h after treatment with 0 or 50 J/m2 of UV. n = 3, mean ± S.D., * indicates p < 0.05 compared with 0 UV by Student's t test. D, immunoblot analysis of ILEI, USF-1, or HSP90 in 501-Mel melanoma cells transduced with lentivirus containing scrambled shRNA or two different sequences targeting USF1.