Figure 2.

β₁Pix is necessary for the inhibition of ENaC by AMPK. A, overlays of typical macroscopic current traces before and after 10-μm amiloride treatment from whole-cell patch-clamped CHO cells expressing mouse ENaC (mENaC) with or without WT β₁Pix or β₁Pix-Δ602–611 either in the presence or absence of pretreatment with AMPK activator (1 mm AICAR, 12 h). Currents were evoked with a voltage ramp (+60 to −100 mV from a holding potential of 40 mV). B, summary graph of the mean ± S.E. for amiloride-sensitive current density at −80 mV for CHO cells expressing mENaC alone or with β₁Pix or β₁Pix-Δ602–611 mutant with or without 1 mm AICAR as indicated. pF, picofarad. C, summary graph of the mean ± S.E. for amiloride-sensitive current density for CHO cells expressing ENaC and either pTracer (control), DN AMPK-α1-K45R, or CA AMPK-γ1-R70Q with or without β₁Pix-Δ602–611. p values are shown for the indicated comparisons.