Figure 5.
β1Pix knockdown blunts ENaC inhibition by AMPK. A and B, mCCDcl1 cells were stably transduced with either a scrambled control shRNA (A) or shRNA directed against β1Pix (B). EVOM studies were performed on polarized cells before and after AA treatment for 4 and 24 h (*, p < 0.05, ENaC current differences of AA-treated cells versus nontreatment controls at the indicated time points). C, comparisons of the percent change in ENaC current following 4 and 24 h of AA treatment between control and β1Pix knockdown cells. β1Pix knockdown significantly reduced the ENaC current inhibition induced by treatment with AMPK activators. D, after equivalent short-circuit current measurements, cells were lysed to verify the knockdown efficiency of β1Pix by immunoblotting. Data shown are mean ± S.E. Asterisks indicate significant difference versus the control treatment at the same time point. p values are also indicated for comparisons in the absence (−) or presence (+) of β1Pix knockdown.