Figure 5.

Multiple sequence alignment of S. enterica AadA (Q8ZPX9; UniProt accession numbers in parentheses) with three characterized ANT(3″)(9) enzymes from E. faecalis (Q71UU1), S. marcescens (Q8VQN7) and P. aeruginosa (Q9RGC2); three ANT(9) enzymes from E. faecalis (Q07448), S. aureus (P0A0D2), and C. jejuni (Q4VR99); and one enzyme lacking ANT(3″) and ANT(9) activity from C. jejuni (Q4VR96). Secondary structure of AadA is indicated above the alignment, colored as in Figs. 2 and 3. Colored dots indicate contacts with ligands; corresponding sequence conservations are marked in the alignment. Green, residues in N-terminal domain coordinating Mg²⁺ and ATP; orange, residues in C-terminal domain coordinating the phosphates of ATP; cyan, residues coordinating rings B and C of streptomycin; yellow, residues coordinating ring A of streptomycin.