Fig. 3.

Liver-specific inactivation of Blnc1 alleviates diet-induced obesity. a A schematic of the strategy for the generation of Blnc1 LKO mice using CRISPR/Cas9. Adenoviral expression of sgRNAs in Cas9 transgenic mice results in liver-specific deletion of Blnc1. b Body weight of control (ctrl, filled, n = 8) and LKO (open, n = 11) mice fed HFD for 10 weeks. Data represent mean ± SEM. *p < 0.01, ctrl vs. LKO, two-way ANOVA with multiple comparisons. c Blood glucose (left) and plasma insulin (right) concentrations in ctrl (n = 9) and LKO (n = 9) mice after HFD feeding for 17 weeks (fast) and 19 weeks (fed). d, e GTT (d) and ITT (e) in ctrl (filled, n = 9) and LKO (open, n = 9) mice fed HFD for 8 and 9 weeks, respectively. Data represent mean ± SEM. **p < 0.01, ***p < 0.001; ctrl vs. LKO, two-way ANOVA with multiple comparisons. f Plasma parameters in mice fed HFD for 19 weeks. Data in (c) and (f) represent mean ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001; ctrl vs. LKO, two-tailed unpaired Student’s t-test