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. 2018 Jul 30;9:2986. doi: 10.1038/s41467-018-05383-2

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — Liver-specific inactivation of Blnc1 attenuates hepatic lipogenesis and preserves metabolic health. a Liver weight, TAG, and cholesterol content in ctrl (filled, n = 9) and LKO (open, n = 9) mice fed HFD for 19 weeks. b H&E staining of liver sections. Scale bar = 100 μm. c Immunoblots of total liver lysates and liver nuclear extracts. d qPCR analysis of hepatic gene expression. Data in (a) and (d) represent mean ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001; ctrl vs. LKO, two-tailed unpaired Student’s t-test