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. 2018 Jul 30;9:2986. doi: 10.1038/s41467-018-05383-2

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 9 — Physical and functional interaction between Blnc1, LXRα, and EDF1. a Diagram of Blnc1 RNA domains (RD, left) and truncation mutants (right). b, c Immunoblots of total lysates and streptavidin (SA) beads precipitated proteins from transiently transfected HEK293T cells. d IP/qPCR analyses of Blnc1 in α-HA immunocomplexes (right) and input (left) from transiently transfected HEK293T cells. e Immunoblots of total lysates and α-Flag immunocomplexes from transiently transfected HEK293T cells. f Immunoblots of total lysates and α-LXRα immunocomplexes from WT and Blnc1 KO hepatocyte transduced with HA-LXRα and HA-EDF1 adenoviruses. g Srebp1c-luciferase reporter assay. Data in (d) and (g) represent mean ± SD (n = 3). ***p < 0.001, vs. vector, one-way ANOVA. h ChIP assay of LXRα occupancy in the livers from WT (open) and KO (filled) mice gavaged with T1317 for 4 days. Data represent mean ± SD (n = 3). **p < 0.01, two-tailed unpaired Student’s t-test