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. 2018 Jul 30;8:11436. doi: 10.1038/s41598-018-29706-x

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Venn diagrams for global methylation based on relative levels at randomly sampled at 5′- CCGG -3′ sites with the MSAP marker in apple skin tissue (Supplementary Table 5). Methylation was analyzed at harvest, prior to scald visualization (pre-symptomatic period; 0 d at 20 °C), and during scald development (symptomatic period; 4 d at 20 °C). Additional experimental details as described in Fig. 1. The percentages of each methylation status (de novo methylation, demethylation and unchanged) from harvest, control and DPA/1-MCP treatments are also shown. Pooled samples from three independent skin tissues were analyzed for each (three) biological replicates. Data represent mean values from three technical replications for each biological sample. Values that significantly different (P < 0.05) from the harvest/control are marked with an asterisk (*).