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. 2018 Jul 30;9:2982. doi: 10.1038/s41467-018-05384-1

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — Immunogenicity of liposomal formulations. a IgG titrations. Absorbance in the ELISA plate versus serum dilution and antibody titer reported as log (EC50). mPEG2000-DSPE, ^LCDX-PEG3400-DSPE, and ^DCDX-PEG3400-DSPE were used as antigens for sLip, ^LCDX-sLip, and ^DCDX-sLip, respectively. b Absorbance in the ELISA plate for anti-PEG IgM evaluation. mPEG2000-DSPE was used as antigen for all formulations. n = 3, data are means ± s.d. Statistical significances were calculated by Student’s t-test. **p < 0.01, ***p < 0.001