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. 2018 Jul 30;8:11384. doi: 10.1038/s41598-018-29885-7

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Effect of pannexin-1 inhibitors on TSPO ligands-induced ATP release. RBCs were incubated at 37 °C, 10% final haematocrit. Carbonexolone (CBX, 10 µM) or Probenecid (PBC, 10 µM) were added to human red blood cells (RBCs), alone or before the addition of 30 µM TSPO ligands (Ro5-4864, panel A; NCS1018, panel B; TRO19622, panel C) or adrenergic cocktail 3 V (panel D). Values are the ratio between extracellular ATP (ATPe) levels after and before the addition of different ligands. Results are expressed as means ± SEM and considered different from basal (vehicle, unstimulated) values when **p < 0.01, n = 7. When indicated, differences were significant compared to stimulated condition.