Figure 7.

TSPO ligands induced Ca2+ entry in red blood cells. Human red blood cells (RBC) were loaded with the Ca²⁺ probe Fluo4/AM and stimulated with TSPO ligand Ro5-4864 (30 µM) in the presence or in the absence of the VDAC inhibitor Bcl-XLBH4 (10 µM). Control experiments were performed adding equivalent volume of ligand vehicle. Ca²⁺ variations (%) were calculated as the fluorescence ratio (Δfluorescence, Ex: 488 nm; Em: 506 nm) after/before the addition of Ro5-4864. Results are expressed as mean ± SEM and considered different from unstimulated (vehicle) or from stimulated values when *p < 0.05; **p < 0.01, n = 3.