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. 2018 Jul 30;8:11401. doi: 10.1038/s41598-018-29837-1

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Large-scale and small-scale phosphoproteomics using patient-derived organoids. (A) Workflow of large-scale and small-scale phosphoproteomics. (B) Picture of a patient-derived organoid. (C) Proportional Venn diagram of class 1 phosphosites identified from patient-derived organoids with fractionation. (D) Proportional Venn diagram of class 1 phosphosites identified from patient-derived organoids in a one-shot analysis. (E) Correlation matrix of phosphosites quantified in all triplicate fractionated and one-shot phosphoproteomics experiments.