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. 2018 Jul 30;8:11429. doi: 10.1038/s41598-018-29870-0

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Trypan blue assay of HCT116 cells irradiated with green laser. Cells were incubated with 8.2 nM AuNPs@PEG for 4 h and medium was replaced. Later, they were irradiated with a power of 3.44 W.cm⁻² for different exposure times and trypan blue assay was performed. For more information see Fig. S9. Cells stain with trypan blue for exposure times greater than 60 s.