Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2018 Jul 30;8:11404. doi: 10.1038/s41598-018-29276-y

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Differential scanning fluorimetry indicates that MCG1516A can interact directly with NBD1. (A) Representative melting curves for human NBD1 determined in the presence of increasing ATP concentrations. (B) Representative F508del-NBD1 melting curve for human NBD-1 in the presence of VX-809 (1 µM) (C) Unfolding of F508del-NBD1 is unaffected by the presence of latonduine. (D) Representative F508del-NBD1 melting curve in the presence of MCG1516A. Also shown is the signal obtained with MCG1516A and Sypro orange dye alone (no protein). (E) Effect of buffer, 1% DMSO or 10 μM MCG1516A on F508del-CFTR stability in the presence of increasing amounts of ATP. In all parts of the figure the error bars indicate SEM (with an n = 3).