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. 2018 Jul 9;53(3):1043–1054. doi: 10.3892/ijo.2018.4477

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Copyright: © Wang et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Biological effects of NT21MP and miR-155-5p inhibitor on PR cells. (A) Effects of NT21MP and miR-155-5p inhibitor on cell migration and invasion were measured using the wound-healing assay. (B) Effects of NT21MP and miR-155-5p inhibitor on cell cycle were analyzed using PI staining and flow cytom-etry. (C) Effects of NT21MP and miR-155-5p inhibitor on cell apoptosis were evaluated using the Annexin V/PI staining and flow cytometry. (D) Reverse transcription-quantitative polymerase chain reaction analysis was used to detect the effect of NT21MP and miR-155-5p inhibitor on the mRNA levels of target gene, cell cycle, and apoptosis-related factors. (E) Western blot analysis results of the protein levels of target genes, cell cycle, and apoptosis-related factors in PR cells were consistent with the mRNA results. Data are presented as the mean ± standard deviation of three independent experiments. ^@@P<0.01, compared with the control group; ^*P<0.05, ^**P<0.01 and ^***P<0.001, compared with SDF-1α treatment; ^###P<0.001, compared with S + NT21MP treatment; ^∆P<0.05 and ^∆∆∆P<0.001, compared with S + 5p inhibitor treatment. NT21MP; 21-residue peptide derived from viral macrophage inflammatory protein II; miR, microRNA; S/SDF-1α, stromal cell-derived factor-1α; NT, NT21MP; PR, paclitaxel-resistant; Bcl-2, B-cell lymphoma 2; Bax, Bcl-2-associated X protein; TP53INP1, tumor protein 53-induced nuclear protein 1; PI, propidium iodide.

Biological effects of NT21MP and miR-155-5p inhibitor on PR cells. (A) Effects of NT21MP and miR-155-5p inhibitor on cell migration and invasion were measured using the wound-healing assay. (B) Effects of NT21MP and miR-155-5p inhibitor on cell cycle were analyzed using PI staining and flow cytom-etry. (C) Effects of NT21MP and miR-155-5p inhibitor on cell apoptosis were evaluated using the Annexin V/PI staining and flow cytometry. (D) Reverse transcription-quantitative polymerase chain reaction analysis was used to detect the effect of NT21MP and miR-155-5p inhibitor on the mRNA levels of target gene, cell cycle, and apoptosis-related factors. (E) Western blot analysis results of the protein levels of target genes, cell cycle, and apoptosis-related factors in PR cells were consistent with the mRNA results. Data are presented as the mean ± standard deviation of three independent experiments. ^@@P<0.01, compared with the control group; ^*P<0.05, ^**P<0.01 and ^***P<0.001, compared with SDF-1α treatment; ^###P<0.001, compared with S + NT21MP treatment; ^∆P<0.05 and ^∆∆∆P<0.001, compared with S + 5p inhibitor treatment. NT21MP; 21-residue peptide derived from viral macrophage inflammatory protein II; miR, microRNA; S/SDF-1α, stromal cell-derived factor-1α; NT, NT21MP; PR, paclitaxel-resistant; Bcl-2, B-cell lymphoma 2; Bax, Bcl-2-associated X protein; TP53INP1, tumor protein 53-induced nuclear protein 1; PI, propidium iodide.