Figure 7.

GNL1 interaction with RPS20 is critical for cell survival. (a) Colony forming assay was carried out in HCT116^p53+/+ cells by expressing GNL1^1–607 or GNL1^{1–607_R22K23A} or RPS20-FLAG alone or in combination. After 48 hours of transfection, GFP positive cells were sorted and selected in cell growth medium containing G418 for 14 days. (b) Colony forming ability of HCT116^p53+/+ cells were determined by depleting GNL1 and/or RPS20 using specific shRNAs with or without GNL1^1–607 or RPS20 expression. The mean surviving fraction of HCT116^p53+/+ cells expressing indicated proteins was calculated and plotted as described in Materials and Methods. Expression of all indicated proteins were determined by western blot analysis using respective antibodies. The full-length blots are given in Supplementary Fig. 13.