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. 2018 Jul 6;53(3):949–960. doi: 10.3892/ijo.2018.4473

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Copyright: © Jia et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Effects of pimozide on the migration of B16 cells. (A) Wound-healing assay. Cells were seeded onto 6-well plates and the cell monolayer was wounded manually. Images were captured at 0, 24 and 48 h following treatment with pimozide. (B) Quantification of the wound-healing assay. Western blotting was conducted to detect MMP2 expression in B16 cells following various concentrations of pimozide for (C) 24 and (D) 48 h. (E) Semi-quantitative results of relative MMP2 protein levels. Values are presented as the means ± standard deviation (n=3, in triplicate). ^**P<0.01 vs. the control group. MMP2, matrix metalloproteinase 2.