Figure 5.

Effects of pimozide and L-MT treatment on proliferation and apoptosis in vivo. B16 cell-bearing mice were treated with pimozide, L-MT or a combination of the two drugs. After 1 week, the mice were sacrificed and tumours were excised. (A) Haematoxylin and eosin staining was conducted to observe tumour tissue morphology. (B) Apoptosis of tumour cells was detected by TUNEL staining. (C) Quantitative analysis of apoptotic cells using the TUNEL assay. (D) Western blotting was performed to detect the expression levels of related proteins. (E) Semi-quantitative results of relative protein levels. n=9/group. ^*P<0.05; ^**P<0.01. IDO, indoleamine 2, 3-dioxygenase; L-MT, L-methyl-tryptophan; p-, phosphorylated; PCNA, proliferating cell nuclear antigen; Stat, signal transducer and activator of transcription; TUNEL, terminal deoxynucleotidyl transferase dUTP nick end-labelling.