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. 2018 Jun 29;53(3):987–1000. doi: 10.3892/ijo.2018.4460

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Copyright: © Zhu et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Effects of treatment with EMCL on caspase-dependent apoptosis. 786-0 and Caki-1 cells were treated with EMCL at the indicated doses. (A) Following treatment for 24 h, levels of apoptosis were determined by FACS analysis, (B) and the percentage of apoptotic cells was calculated. (C) Protein expression levels of cleaved caspase-3, cleaved caspase-9, cleaved caspase-PARP, and BCL-2/BAX in 786-0 and Caki-1 cells were analyzed by western blot analysis. (D) Ratio of Bax/Bcl-2. (E) Quantitative analysis of proteins. (F) Release of cytochrome c from the mitochondria to the cytoplasm was observed by immunofluorescence imaging analysis in 786-0 and Caki-1 cells (magnification, ×630). Data are presented as the mean ± standard deviation of three independent experiments. ^*P<0.05 and ^**P<0.01, vs. dimethyl sulfoxide-treated group. EMCL, epoxymicheliolide; PARP, poly (ADP-ribose) polymerase ; Bcl-2, B-lymphoma 2; Bax, Bcl-2-assocated X protein; Cyto C, cytochrome c.