Table 2. Observed and predicted values for optimizing the hydrolysis condition.

Trial	Conditionsa	DH		DPPH		ABTS
		obs	pre	obs	pre	obs	pre
1	x1 = 10%, x2 = 18 h	48.9	47.5	79.1	81.7	81.2	77.9
2	x1 = 15%, x2 = 12 h	46.7	49.6	94.9	88.8	82.3	82.5
3	x1 = 15%, x2 = 24 h	40.1	44.3	78.4	83.4	82.2	86.6
4	x1 = 20%, x2 = 12 h	45.0	47.3	86.4	84.9	77.1	77.6
5	x1 = 20%, x2 = 24 h	43.1	40.8	79.0	77.6	78.0	78.7

Notes.

obs

observed

pre

predicted

^a x₁ represents enzyme concentration. x₂ represents hydrolysis time.