Table 3. Amino acid composition of crude-MMPH and its ultrafiltrated peptide fractions.
Content (mg / g Protein)a | Total amino acid(% w/w Protein)a | |||||||
---|---|---|---|---|---|---|---|---|
Group | Amino acid | Total amino acids | Free amino acids | Amino acid in peptide form | F1 | F2 | F3 | F4 |
Acidic (−) | Asp | 104.39 ± 3.93 | 1.06 ± 0.04 | 103.33 ± 3.89 | 14.23 ± 0.15 | 13.84 ± 1.54 | 13.74 ± 0.57 | 11.77 ± 0.80 |
Glu | 162.66 ± 2.044 | 16.92 ± 0.51 | 145.70 ± 2.55 | 18.30 ± 0.02 | 17.95 ± 0.23 | 18.23 ± 0.95 | 18.72 ± 0.95 | |
Basic (+) | Lys | 72.11 ± 6.12 | 28.94 ± 0.20 | 43.17 ± 5.93 | 8.15 ± 0.19 | 8.32 ± 0.43 | 8.00 ± 0.64 | 6.89 ± 0.45 |
Arg | 67.00 ± 1.39 | 22.10 ± 0.27 | 44.91 ± 1.12 | 6.63 ± 0.57 | 6.71 ± 0.55 | 6.72 ± 0.26 | 6.85 ± 0.47 | |
His | 20.01 ± 0.76 | 11.58 ± 0.59 | 8.81 ± 0.16 | 3.55 ± 0.66 | 3.07 ± 0.69 | 2.75 ± 0.55 | 3.02 ± 0.56 | |
Hydrophobic | Gly | 36.78 ± 2.52 | 5.49 ± 0.86 | 31.29 ± 3.38 | 3.19 ± 0.45 | 3.78 ± 0.48 | 3.73 ± 1.07 | 3.98 ± 0.20 |
Ala | 40.02 ± 1.26 | 10.36 ± 0.23 | 29.66 ± 1.03 | 4.12 ± 0.28 | 3.97 ± 1.03 | 3.91 ± 0.80 | 4.88 ± 0.57 | |
Val | 36.48 ± 1.50 | 6.68 ± 0.25 | 29.80 ± 1.75 | 4.48 ± 0.54 | 5.18 ± 0.45 | 5.30 ± 0.45 | 5.02 ± 0.95 | |
ILe | 36.86 ± 1.84 | 3.63 ± 0.17 | 33.23 ± 2.01 | 4.72 ± 0.55 | 4.64 ± 0.69 | 4.67 ± 2.06 | 3.56 ± 0.95 | |
Leu | 62.93 ± 0.15 | 22.29 ± 0.14 | 40.64 ± 0.11 | 6.48 ± 0.28 | 6.63 ± 0.91 | 6.70 ± 0.74 | 8.60 ± 0.74 | |
Pro | 48.01 ± 1.09 | 3.57 ± 0.10 | 44.44 ± 0.99 | 4.97 ± 0.22 | 5.59 ± 1.19 | 6.17 ± 0.26 | 3.62 ± 0.71 | |
Met | 8.90 ± 1.39 | 4.60 ± 0.11 | 4.30 ± 1.50 | 1.00 ± 0.21 | 1.13 ± 0.37 | 1.09 ± 0.49 | 1.60 ± 0.54 | |
Cys | 3.97 ± 0.34 | 0.19 ± 0.23 | 3.78 ± 0.57 | 2.27 ± 0.18 | 0.83 ± 0.64 | 0.60 ± 0.54 | 0.30 ± 0.16 | |
Aromatic | Tyr | 29.03 ± 2.72 | 5.24 ± 1.15 | 23.79 ± 1.58 | 3.73 ± 0.08 | 3.37 ± 0.62 | 3.44 ± 0.57 | 3.81 ± 0.57 |
Phe | 45.61 ± 1.39 | 7.06 ± 0.16 | 38.55 ± 1.54 | 4.73 ± 0.23 | 5.08 ± 0.43 | 5.17 ± 1.78 | 6.58 ± 0.93 | |
Trp | 6.05 ± 0.31 | 1.18 ± 0.17 | 4.87 ± 0.48 | 0.70 ± 0.04 | 0.74 ± 0.40 | 0.78 ± 0.76 | 0.83 ± 0.70 | |
Hydrophilic | Ser | 54.27 ± 2.38 | 13.70 ± 0.88 | 40.57 ± 3.25 | 5.48 ± 0.29 | 5.76 ± 1.03 | 5.67 ± 0.79 | 6.50 ± 0.76 |
Thr | 33.41 ± 1.71 | 5.93 ± 0.27 | 27.48 ± 1.44 | 3.34 ± 0.35 | 3.46 ± 0.33 | 3.37 ± 0.06 | 3.52 ± 0.76 | |
Total | 868.51 | 170.54 | 697.98 | 100 | 100 | 100 | 100 |
Notes.
Each value is expressed as the mean ± SD (n = 3).