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. Author manuscript; available in PMC: 2019 Sep 1.
Published in final edited form as: Biomaterials. 2018 Feb 13;178:751–766. doi: 10.1016/j.biomaterials.2018.01.032

Figure 3.

Figure 3.

A) Confocal image of mal-546 gradients in HANDBF/PEG with scanning intervals either 20 or 5 μm apart in the z-axis. Gradients were formed using a scan speed of 0.009 μm μs−1. Confocal images were enhanced for visualization. B) Quantification of mal-546 gradients in HANDBF/PEG with scanning intervals either 20 or 5 μm apart in the z-axis. Background mal-546 concentration was subtracted from the immobilized mal-546 concentration. C) Quantification of low, medium, and high (0.7242, 0.8624, and 1.2074 ng mL−1 μm−1, respectively) EGF555 gradients in HANDBF/MMPx hydrogels created at scan speeds of 0.527, 0.263, and 0.132 μm μs−1, respectively. At the surface of the hydrogel (0 μm) the hydrogel was scanned one time. The scan number was increased by one scan every 5 μm interval going into the hydrogel until a gradient of 150 μm in depth was formed. The amount of EGF555 in the non-patterned regions of the hydrogel was also quantified (0.1581 ng mL−1 μm−1). Slopes were found to be significantly different (****p<0.0001). Error bars displayed as SEM, n=4. D) Confocal images of low, medium, and high EGF555 gradients in HANDBF/MMPx hydrogels.